Gene description for SPAG1
Gene name sperm associated antigen 1
Gene symbol SPAG1
Other names/aliases CILD28
CT140
DNAAF13
HEL-S-268
HSD-3.8
SP75
TPIS
Species Homo sapiens
 Experiment description of studies that identified SPAG1 in Breast cancer cells

1
Experiment ID1759
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 35918900   
OrganismHomo sapiens
Experiment descriptionCharacterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
AuthorsBauzá-Martinez J, Armony G, Pronker MF, Wu W.
Journal name J Extracell Vesicles
Publication year2022
SampleBreast cancer cells
Sample nameLM2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectometry
EV-TRACK -

2
Experiment ID1761
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 35918900   
OrganismHomo sapiens
Experiment descriptionCharacterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
AuthorsBauzá-Martinez J, Armony G, Pronker MF, Wu W.
Journal name J Extracell Vesicles
Publication year2022
SampleBreast cancer cells
Sample nameLM2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectometry
EV-TRACK -

3
Experiment ID3350
Identified moleculeprotein
Extracellular vesicle typeSupermeres
MISEVEV-like nanoparticles
Identification method Mass spectrometry
PubMed ID 34887515   
OrganismHomo sapiens
Experiment descriptionSupermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
AuthorsZhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year2021
SampleBreast cancer cells
Sample nameMDA-MB-231
Isolation/purification methodsDifferential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
ELISA
EV-TRACK -

<